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J Shahrekord Univ Med Sci. 2021;23(4): 162-167.
doi: 10.34172/jsums.2021.27
  Abstract View: 43
  PDF Download: 32

Original Article

The study of P-glycoprotein A, G-glutamylcysteine synthetase 1, and aquaglyceroporin 1 genes expression in non-healing zoonotic cutaneous leishmaniasis cases

Seyed Hossein Hejazi 1 ORCID logo, Sedigheh Saberi 1 ORCID logo, Reza Arjmand 2 ORCID logo, Simindokht Soleimanifard 3* ORCID logo

1 Skin Disease and Leishmaniasis Research Center, Department of Parasitology, School of Medicine Isfahan University of Medical Sciences and Health Services, Isfahan, Iran
2 Department of Parasitology, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran
3 Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran
*Corresponding Author: Simindokht Soleimanifard, Parasitology and Mycology Department, School of Medicine, Isfahan University of Medical Science, Hezar Jerib Street, Isfahan, Iran. Tel: +989132943369, Email: Soleimanifard@med.mui.ac.ir

Abstract

Background and aims: Regarding the increasing numbers of clinical antimonial-resistant Leishmaniasis, understanding the reasons for drug resistance is helpful. This study aimed to find the expression level of the genes related to resistance, P-glycoprotein A (PgpA), G-glutamylcysteine synthetase 1 (Gsh1), and aquaglyceroporin 1 (Aqp1) in antimonial-resistant clinical isolates.

Methods: Samples were isolated from leishmaniasis ulcers of 10 non-healing patients and the species were identified by the nested-polymerase chain reaction (PCR) method. In vitro experiments were performed using the amastigote-macrophage model by J774 cell line, and in vivo studies were conducted by animal model, the Balb/c mice. Finally, the values of genes expression were determined by quantitative-reverse transcription (q-RT) real-time PCR method and then compared with non-resistant Leishmania major (MRHO/IR/75/ER).

Results: Molecular identification showed that all isolated protozoa were L. major. The isolated samples from clinical resistant patients represented no increase in expression in the tested resistance genes (P>0.05). Finally, it was found that the lack of improvements in patients was not associated with the increased expression of resistance genes.

Conclusion: In general, no inherent resistance was observed in the tested samples neither a correlation between the healing of lesions and the level of genes expression.


Keywords: Drug resistance, Gene expression, Zoonotic cutaneous leishmaniasis, q-RT PCR

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Submitted: 17 Feb 2021
Accepted: 03 May 2021
ePublished: 06 Dec 2021
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