J Shahrekord Univ Med Sci. 2020;22(4): 173-180.
doi: 10.34172/jsums.2020.28
  Abstract View: 938
  PDF Download: 486

Original Article

Characterization of the decellularized ovine pericardium for skin tissue engineering

Morteza Alizadeh 1 ORCID logo, Leila Rezakhani 1 ORCID logo, Mohammad khodaei 2 ORCID logo, Akram Alizadeh 3* ORCID logo

1 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Department of Materials Science and Engineering, Golpayegan University of Technology, Golpayegan, Iran
3 Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding Author: Email: alizadehbio@gmail.com


Background and aims: Some biological scaffolds are used as appropriate skin substitutes, including decellularized amniotic membrane or dermis although the ovine pericardial tissue has not been characterized or used for skin tissue engineering. In this regard, this study focused on the decellularization and characterization of ovine pericardium for skin tissue engineering.

Materials and Methods: To this end, two different methods were used for decellularization, including safety data sheet (SDS) 1% (method 1) and Triton X-100 1% (method 2). In addition, histological examinations (H & E staining), DNA content assay, scanning electron microscopy (SEM), MTT test using human adipose-derived mesenchymal stem cells, and tensile tests were conducted for sample characterization.

Results: Based on the results, the DNA content showed significant DNA removing (P<0.001) after decellularization with methods 1 and 2 in comparison to native tissues although the significance level between the two methods was P=0.06. In the SEM examination, cells were effectively removed while the extracellular matrix remained intact in both groups. Based on the results of the MTT test, the toxicity was not significant (P=0.36). On the other hand, mechanical property assay revealed a higher value of Young’s modulus in method 1 (34.12 MPa) compared to Method 2 (32.57 MPa) and native tissues (30 MPa). Finally, the highest strain at the break point (approximately 0.6) belonged to the native sample.

Conclusion: In general, the ovine seems to be a good alternative for skin tissue engineering and regeneration since it is a post-slaughtering waste tissue has low thickness, is wide and spread, and easy to be decellularized with SDS 1% and Triton X-100. Eventually, it has good properties for cell seeding based on the findings of our study and the capability of vascularization reported in the literature.

First Name
Last Name
Email Address
Security code

Abstract View: 939

Your browser does not support the canvas element.

PDF Download: 486

Your browser does not support the canvas element.

Submitted: 13 Mar 2020
Accepted: 26 Apr 2020
ePublished: 29 Dec 2020
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)