J Shahrekord Univ Med Sci. 2024;26(1): 1-6.
doi: 10.34172/jsums.673
  Abstract View: 306
  PDF Download: 247

Original Article

Comparative evaluation of the serum biochemical and lipid profile changes in spayed male and female rats

Abdolrasoul Namjou 1* ORCID logo, Esfandiar Heidarian 2 ORCID logo, Abuzar Alikhani 3 ORCID logo

1 Department of Pathology, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2 Department of Biochemistry, Clinical Biochemistry Research Center, Shahrekurd University of Medical Sciences, Shahrekord, Iran
3 Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
*Corresponding Author: Abdolrasoul Namjou, Email: ar.namjo72@gmail.com


Background and aims: This study investigated the effects of gonadal removal in male and female rats on changes in serum biochemical parameters.

Methods: Twenty-eight adult male and female rats were divided into four groups of 7 animals for a period of 9 weeks. The first and second groups of intact male and female rats, as well as the third and fourth groups of male and female rats, were gonadectomized, respectively. At the end of the ninth week of the study, the rats were anesthetized with chloroform, and the amount of glucose, some lipid parameters in serum, and the activity of a number of serum enzymes were measured after taking blood from the heart.

Results: Alanine aminotransferase, and alkaline phosphatase (ALP) levels were higher in intact male rats than in intact female rats, respectively (P=0.03, P=0.015). The amounts of glucose and cholesterol of low-density lipoprotein (LDL) in intact female rats were higher than in the ovariectomized rats, respectively (P=0.07, P=0.039, P=0.03). The amount of lactate dehydrogenase (LDH) in spayed female rats demonstrated a significant increase compared to other groups (P=0.001). However, there was a significant decrease in calcium levels in gonadectomized rat groups in comparison to intact female rats (P=0.02). Finally, a significant increase was found in phosphorous levels in the intact male rats group compared to other groups (P=0.002).

Conclusion: Decreased sex hormones in gonadectomized rats compared with intact rats could lead to increased serum levels of LDH, ALP, and LDL cholesterol that may result in the development of metabolic and atherogenesis syndromes and acute liver failure.

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Abstract View: 307

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Submitted: 14 Apr 2022
Revision: 19 Dec 2022
Accepted: 19 Dec 2022
ePublished: 31 Jan 2024
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