J Shahrekord Univ Med Sci. 2023;25(2): 90-96.
doi: 10.34172/jsums.2023.744
  Abstract View: 270
  PDF Download: 229

Original Article

The effect of 12 weeks of aerobic exercise and caloric restriction on Nrf2 protein expression in non-alcoholic fatty liver disease in rats

Farzaneh Yadegari 1* ORCID logo, Farhad Rahmani Nia 2 ORCID logo

1 PhD Student of Sports Physiology, Faculty of Physical Education and Sports Sciences, Gilan University, Rasht, Iran.
2 Professor of Sports Physiology Department, Faculty of Physical Education and Sports Sciences, Gilan University, Rasht, Iran.
*Corresponding Author: Corresponding Author: Farzaneh Yadegari, Email: , Email: farzan.yadegar@gmail.com


Background and aims: One of the most common causes of liver disease is non-alcoholic fatty liver disease (NAFLD), and its prevalence along with serious clinical problem is a growing. No studies have been conducted on the effect of calorie restriction (CR) and aerobic exercise (AE) on nuclear factor erythroid-related factor 2 (Nrf2) in rats with NAFLD. The present study aimed to assess the effects of 12 weeks of CR and AE on Nrf2 protein expression in rats with NAFLD.

Methods: A total of 40 rats participated in this study, and 32 of them developed NAFLD by feeding fatty food (34% fat, 19% protein, and 47% carbohydrates) for eight weeks. Rats were classified into five groups: sham, control, CR, AE, and calorie restriction-aerobic exercise (CA). First, 60% of the daily diet was given to the CR and CA groups. AE was done for 12 weeks, five sessions per week on a treadmill for rats. Oil red, hematoxylin-eosin (H & E) staining, and protein expression levels in the groups were evaluated. To analyze the data, one-way ANOVA was used at a significance level of P<0.05.

Results: The results showed a significant difference between the liver fat of the control group and other groups (RC: P=0.001, AE: P=0.001, RA: P=0.001). In healing liver damage, the control group was significantly different from the CA group (P=0.002). Regarding the Nrf2 protein expression, the CA group had significantly higher expression than the CR group (P=0.028), however there was no significant difference between the CA and AE groups (P=0.44).

Conclusion: The findings revealed that AE through CR can cause recovery for NAFLD.

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Submitted: 07 Jul 2022
Revision: 12 Sep 2022
Accepted: 13 Sep 2022
ePublished: 10 Dec 2022
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