Logo-jskums
J Shahrekord Univ Med Sci. 2024;26(3): 94-100.
doi: 10.34172/jsums.816
  Abstract View: 94
  PDF Download: 74

Original Article

Combination of medicinal plants with antibiotics against Klebsiella pneumoniae and Acinetobacter baumannii

Vahid Reisi-Vanani 1 ORCID logo, Abolfazl Gholipour 2 ORCID logo, Sajad Maghareh-Dehkordi 3 ORCID logo, Zahra Lorigooini 4* ORCID logo

1 Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Cellular and Molecular Research Center, Basic Health Sciences Institute, Department of Microbiology and Immunology, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Student Research Committee, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
4 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding Author: Zahra Lorigooini, Email: zahralorigooini@gmail.com

Abstract

Background and aims: One essential plant-based strategy to deal with infections is antimicrobial synergism, which can make antimicrobials more efficient. The aim of this study was to investigate the interaction between these extracts and two widely used antibiotics, meropenem and gentamicin, on two multidrug-resistant (MDR) bacteria, K. pneumoniae and A. baumannii, in vitro.

Methods: Different concentrations of Rosa damascena Mill., Malva sylvestris L., and Zataria multiflora Boiss. hydroalcoholic extracts (2-fold serial dilution from 131072 to 256 μg/mL) were administered against two MDR bacteria, and their combination with gentamicin and meropenem (serial dilution from 32 to 0.015 μg/mL) was investigated by the resazurin-based microdilution and the checkerboard method. The phytochemical properties of the extracts were also examined, and the total phenolic content (TPC), total flavonoid content (TFC), anthocyanin content, and antioxidant capacity of the extracts were determined.

Results: Z. multiflora and R. damascena showed high antibacterial activity, and their minimal inhibitory concentrations on Acinetobacter baumannii were 1024 and 2048 μg/mL, respectively. Z. multiflora also had high TFC, TPC, and antioxidant activity and demonstrated additive interaction with meropenem and gentamicin with fractional inhibitory concentrations of 1 and 0.75, respectively.

Conclusion: We suggest the potency of Z. multiflora-antibiotic combinations in treating MDR A. baumannii after future clinical studies.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 95

Your browser does not support the canvas element.


PDF Download: 74

Your browser does not support the canvas element.

Submitted: 28 Oct 2022
Accepted: 17 Dec 2022
ePublished: 29 Sep 2024
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)