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J Shahrekord Univ Med Sci. 2024;26(4): 172--184.
doi: 10.34172/jsums.960
  Abstract View: 33
  PDF Download: 31

Original Article

Phytochemical contents, in vitro and in vivo antioxidant and anticancer activities of avocado (Persea americana Mill.) seed extract against MCF-7 and HMVII cancer cells

Cletus Anes Ukwubile* ORCID logo, Aishat Omolabake Makinde ORCID logo

1 Department of Pharmacognosy, Faculty of Pharmacy, University of Maiduguri, Maiduguri, Nigeria
*Corresponding Author: Cletus Anes Ukwubile, Email: caukwubile@unimaid.edu.ng, Email: doccletus@yahoo.com

Abstract

Background and aims: Avocados are unique in Nigeria for their taste and medicinal uses. This study was performed to determine the phytochemicals and evaluate the antioxidant and cytotoxic effects of avocado seed extracts (ASEs).

Methods: The phytochemicals were analyzed using the gas chromatography-mass spectrometry (GC-MS) apparatus. The total phenolic and flavonoid contents and in vitro antioxidant activity were evaluated using standard methods. In contrast, in vivo, antioxidants were used in a group of thirty healthy Wistar rats randomly grouped into five groups of six, with cytotoxicity activity by benchtop and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assays and apoptosis by assessing caspase-3-like activity.

Results: The GC-MS revealed polyphenols and fatty acids. Phenolic and flavonoid contents were 1178.67 mg gallic acid equivalent per gram (GAE/g) and 86.24 mg rutin equivalent per gram (RE/g), respectively. High radical scavenging toward 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS, and ferric reducing antioxidant power (FRAP) reducing power with IC50 values of 25.25±1.01 µg/mL, 38.22±2.01 µg/mL, and 43.99 mM Fe (II) equivalent, respectively, in vitro antioxidation as well as dose-dependent in vivo antioxidant activities with reduced malondialdehyde (MDA), total superoxide dismutase (TSOD), and catalase (CAT) and increased glutathione peroxidase (GSH-Px) contents in vital organs were obtained. Cytotoxic effects on MCF-7 and HMVII cells were detected, with IC50 values of 16.51±0.02 µg/mL and 31.71±2.03 µg/mL, respectively, which was statistically significant (P=0.001) when compared to doxorubicin standard drug and a higher selective index on breast cancer (BC).

Conclusion: The findings revealed that avocado seed possessed antioxidant and anticancer activities on human MCF-7 and HMVII cancer cell lines.


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Submitted: 07 Mar 2024
Accepted: 13 Apr 2024
ePublished: 30 Dec 2024
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