Shahrekord University of Medical Sciences Journal of Shahrekord University of Medical Sciences 2717-0071 21 3 2019 06 01 Comparison of polystyrene versus cycloolefin microplates in absorbance measurements in the UV/VIS region of the spectrum 110 113 10.34172/jsums.2019.19 EN Mehri Abedi https://orcid.org/0000-0002-0945-8486 Reza Ahangari Cohan Mehdi Shafiee Ardestani Fatemeh Davami https://orcid.org/0000-0002-7693-2926 Journal Article 10.34172/jsums.2019.19 2018 10 15 2018 11 20 Background and aims: Polystyrene microplates are generally used to specify the absorption in the visible light region of the spectrum. However, they are capable of absorption in the ultraviolet (UV) range of the spectrum while they are not suitable for UV spectroscopy analysis. This study aimed to compare polystyrene and cycloolefin microplates for their background absorbance characteristics in the UV/ VIS region of the spectrum. Methods: Background absorbance of the mentioned microplates was measured using two different spectrophotometers and four various samples. Results: The analysis of our results verified the advantage of applying cycloolefin microplate over polystyrene one for absorbance measurements in the UV range of the spectrum. Conclusion: In general, suitable microplate selection is a critical factor in absorbance measurements, especially in the UV portion of the spectrum.