Shahrekord University of Medical Sciences Journal of Shahrekord University of Medical Sciences 2717-0071 27 1 2025 04 26 Investigating the effect of royal jelly and nanosilver particles on kidney and liver cytokines 21 25 10.34172/jsums.954 EN Hajar Dahim https://orcid.org/0009-0007-2338-9863 Mohammad Kazemi-Arababadi https://orcid.org/0000-0002-4315-8153 Mohammadmohsen Taghavi https://orcid.org/0000-0003-3247-5484 Zahra Taghipour https://orcid.org/0000-0002-6670-724X Samereh Dehghani-Soltani https://orcid.org/0000-0003-1074-3345 Abdolreza Babaea https://orcid.org/0000-0002-9848-5442 Mohammadreza Rahmani https://orcid.org/0000-0001-7395-5770 Alireza Nazari https://orcid.org/0000-0001-8352-793X Mahsa Dalfardi https://orcid.org/0000-0002-1065-1654 Hossein Pourmobini https://orcid.org/0000-0003-3089-0203 Erfan Shahabinejad https://orcid.org/0000-0002-0204-0394 Ahmad Shabanizadeh https://orcid.org/0000-0002-6510-1190 Journal Article 10.34172/jsums.954 2024 01 28 2024 05 06 Background and aims: Cytokines play crucial roles in immune responses. Nanoparticles, such as nanosilver (NS), are widely used to improve drug delivery. It has been reported that NS may affect immune cell functions. Royal jelly (RJ) has protective roles against several molecules, including free radicals and other harmful molecules. This project was designed to explore the influential role of RJ and NS in the immune response in the livers and kidneys of rats. Methods: In this study, 40 rats were divided into control, RJ, NS, and RJ-NS-treated groups. The rats were treated with RJ, NS, and their combination. Further, levels of interleukin-10 (IL-10), IL-13, tumor necrosis factor-α (TNF-α), and C-C motif chemokine ligand 3 (CCL3) in the liver and kidney were evaluated using the enzyme-linked immunosorbent assay technique. Results: The results demonstrated that NS reduced the CCL3 and TNF-α levels in the liver and kidney, respectively. In addition, RJ decreased IL-13 levels in both kidney and liver tissues. In the kidney, IL-13 levels were significantly reduced in the RJ group compared to the control (P=0.043) and NS-RJ (P=0.002) groups. In the liver, IL-13 levels also decreased in the RJ group compared to the control group (P=0.050). Conclusion: Based on the results, RJ significantly protects macrophage functions, the main cells that produce TNF-α and respond to CCL3. RJ may also improve the pathogenesis of IL-13-related diseases. RJ could neutralize the effects of NS on CCL3 and TNF-α levels.