Journal of Shahrekord University of Medical Sciences
J Shahrekord Univ Med Sci. 2020;22(1): 38-45.
doi: 10.34172/jsums.2020.07
  Abstract View: 78

Original Article

The effects of pBudCE4.1-azurin-MAM-A recombinant vector on IL-2, IL-6, IL-7, and IL-10 expressions in laboratory mice

Payam Ghasemi-Dehkordi 1 ORCID logo, Abbas Doosti 2 * ORCID logo, Mohammad-Saeid Jami 3 ORCID logo

1 Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
2 Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
3 Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
*Corresponding Author: Abbas Doosti, Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran, Postal box: 166, Tel: +98-38-33361001, Fax: +98-38-33361001, Cell phone: +98-913-3838830, E-mail: biologyshki@yahoo.com

Abstract

Background and aims: Breast cancer is one of the most common types of malignancy in women with morbidity and mortality (15.0%) in the world. The antitumor activity of azurin protein produced by Pseudomonas aeruginosa has been described before. Mammaglobin-A (MAM-A) protein is especially expressed in 40%-80% of breast cancer types and this protein is a very specific molecular marker for stimulating the immune system. Accordingly, this study investigated the effects of pBudCE4.1-azurin-MAM-A recombinant vector on the induction of the immune system in laboratory mice by the real-time polymerase chain reaction (PCR) method.

Methods: The pBudCE4.1-azurin-MAM-A recombinant and empty vectors were purchased and then separately transformed into Escherichia coli for multiplying. Next, each plasmid was extracted and the accuracy of transformation was confirmed by the PCR. These recombinant and empty (control) vectors were separately infused into the thigh muscle of the animals and the healthy group was infused with phosphatebuffered saline. The infusion sites, blood specimens, as well as the serum of the animals were collected and examined by serological and molecular tests.

Results: Molecular and serological studies showed that the serum and expression levels of IL-2, IL-6, IL-7, and IL-10 in infused mice with pBudCE4.1-azurin-MAM-A recombinant vector significantly increased compared to healthy animals and injected mice with an empty vector (P<0.05).

Conclusion: In general, the findings revealed that the pBudCE4.1-azurin-MAM-A recombinant vector can stimulate the immune system of the mouse by an increase in the expression levels of IL-2, IL-6, IL-7, and IL-10. Thus, it would be better to examine the effects of this recombinant vector as a DNA vaccine on the prevention and treatment of breast cancer.

Keywords: Azurin, MAM-A, Recombinant vector, Breast cancer
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Submitted: 31 Aug 2019
Accepted: 30 Oct 2019
ePublished: 28 Feb 2020
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