Logo-jskums
J Shahrekord Univ Med Sci. 2021;23(2): 93-98.
doi: 10.34172/jsums.2021.15
  Abstract View: 101
  PDF Download: 67

Original Article

The antinociceptive effects of folic acid using formalin and acetic acid tests in male mice

Morteza Hajiallilo 1 ORCID logo, Saeid Abbasi-Maleki 2,3* ORCID logo

1 Department of Pharmacology and Toxicology, Urmia Branch, Islamic Azad University, Urmia, Iran
2 Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Department of Pharmacology & Toxicology, School of Pharmacy, Kermanshah University of Medical Science, Kermanshah, Iran
*Corresponding Author: Saied Abbasi-Maleki, Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran, Department of Pharmacology & Toxicology, School of Pharmacy, Kermanshah University of Medical Science, Kermanshah, Iran. Tel. +988314276484; fax: +988314276493.Cell phone. +98 9141130054, Email: s.maleki@kums.ac.ir

Abstract

Background and aims: Antidepressant agents such as imipramine are clinically used to control and treat different types of pain, especially neuropathic pain. Studies have shown the antidepressant-like activity of folic acid (FA). This study aimed to investigate the potential antinociceptive effects of FA using formalin and acetic acid tests in male mice.

Methods: Sixty male albino mice (20-30 g) were randomly divided into 10 groups (n=6 in each group) of negative control, positive control (morphine or indomethacin), and FA (10, 15, and 30 mg/kg) groups. In the formalin test, duration of paw licking and biting the right hind paw during acute (0-5 minutes) and chronic (15-60 minutes) pain after intraplantar injection of formalin 2.5% (25 µL) was recorded. In the writhing test, the abdominal constrictions were recorded after the intraperitoneal injection of acetic acid 1%.

Results: Only a high dose (30 mg/kg) of FA significantly reduced acute pain (P=0.001) compared with the control group. But all doses of FA significantly decreased chronic pain (P=0.001). In addition, morphine significantly reduced both phases of pain (P=0.020 and P=0.001, respectively). Moreover, indomethacin and all doses of FA decreased the number of abdominal constrictions induced by acetic acid (P=0.001).

Conclusion: Compared with acute (neurogenic) pain, FA more potently decreases chronic (inflammatory) pain. Furthermore, FA decreases the parietal pain that could potentially represent antinociceptive effect. However, further studies are needed to elucidate the exact mechanism of FA’s analgesic activity.

Keywords: Folic acid, Antinociceptive, Antidepressants, Formalin test, Writhing test, Mice
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 101

Your browser does not support the canvas element.


PDF Download: 67

Your browser does not support the canvas element.

Submitted: 26 Feb 2020
Accepted: 18 Nov 2020
ePublished: 30 Jun 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)