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J Shahrekord Univ Med Sci. 2021;23(2): 81-86.
doi: 10.34172/jsums.2021.13
  Abstract View: 119
  PDF Download: 92

Original Article

Evaluation of isolation method in remaining of differentiation potential of perivascular human umbilical cord mesenchymal stem cells toward male germ cell-like

Ali Shojaeian 1 ORCID logo, Ameneh Mehri-Ghahfarrokhi 2 ORCID logo, Shima Rahmati-Dehkordi 3 ORCID logo, Mehdi Banitalebi-Dehkordi 4* ORCID logo

1 Research Center for Molecular Medicine, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
2 Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
4 Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding Author: Mehdi Banitalebi-Dehkordi, Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Rahmatieh, Shahrekord, Iran. Phone: +983833346692, Email: mehdibanitalebi@ gmail.com

Abstract

Background and aims: Infertility is one of the most common problems among couples. Generation of male germ cells from adult stem cells is a current promising priority of researchers. This study aimed to investigate the potential of human umbilical cord mesenchymal stem cells (hUMSCs) on the expression of male germ cell markers after isolating by this method.

Methods: The hUMSCs was incubated with retinoic acid, testosterone, and conditioned medium (prepared from testicular cell cultures of 7-day-old mice) during 3 days. The bands were visualized and densitometry was accomplished using LI-COR Biosciences software.

Results: The high expression levels of C-KIT, DAZL, PIWIL2, and DDX4 in mRNA and protein levels were observed in treated hUMSCs.

Conclusion: Results of reverse transcription polymerase chain reaction (RT-PCR) and western blotting showed that method of isolation had no adverse effects on differentiation potential of hUMSCs.

Keywords: Infertility, Human umbilical cord mesenchymal stem cell, Male germ cell, Retinoic acid
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Abstract View: 119

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Submitted: 13 May 2020
Accepted: 18 Oct 2020
ePublished: 30 Jun 2021
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