Logo-jskums
J Shahrekord Univ Med Sci. 2021;23(2): 76-80.
doi: 10.34172/jsums.2021.12
  Abstract View: 690
  PDF Download: 425

Original Article

Comparison of ADAM17 gene expression level in acute lymphoblastic leukemia patients

Shakiba Karimi 1 ORCID logo, Noosha Zia Jahromi 1* ORCID logo

1 Department of Biology, Science Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
*Corresponding Author: *Corresponding Author: Noosha Zia Jahromi, Department of Biology, Science Faculty, Islamic Azad University, Shahrekord Branch Shahrekord, Iran. Tel: +9133146504; Email: , Email: nooshazia.59@gmail.com

Abstract

Background and aims: In acute lymphoblastic leukemia (ALL), large numbers of stem cells become lymphoblasts or lymphocytes. Among the genetic factors influencing cancer is the ADAM (a disintegrin and metalloprotease) gene family. Due to the important role of this family in cancer, this study aimed to compare the expression level of ADAM17 gene in patients with ALL and healthy individuals.

Material and Methods: In this case-control study, 40 venous blood samples were taken from ALL patients referred to Omid hospital in Isfahan, Iran. Also, 40 venous blood samples were taken from healthy individuals in vitro. Lymphocyte isolation was performed using a ficol and cell RNA was isolated using an RNX-Plus kit. It was then converted to cDNA using the Yekta Tajhiz Azma kit. Finally, reverse transcription-polymerase chain reaction (RT-PCR) technique was used to evaluate the relative expression of ADAM17 gene in blood samples of healthy individuals and patients with leukemia, and the ratio was measured with the reference GAPDH gene. SPSS software version 22 and t test were used to analyze the data.

Results: The expression level of ADAM17 gene in patients with ALL compared to the control group showed a significant increase, which was statistically significant (P=0.043).

Conclusion: It seems that increasing the expression of ADAM17 gene in people with ALL is a suitable biomarker to diagnose this disease.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 691

Your browser does not support the canvas element.


PDF Download: 425

Your browser does not support the canvas element.

Submitted: 09 Sep 2020
Accepted: 04 Oct 2020
ePublished: 30 Jun 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)