Logo-jskums
J Shahrekord Univ Med Sci. 2021;23(3): 124-130.
doi: 10.34172/jsums.2021.21
  Abstract View: 82
  PDF Download: 58

Original Article

The effect of aerobic exercise training on gene expression of beta3-adrenergic receptor and beta-arrestin2 in inguinal white adipose tissue of mice fed with a high fat diet

Saeed Daneshyar 1* ORCID logo, Mehdi Bahmani 2 ORCID logo, Yazdan Fourotan 3 ORCID logo

1 Assistant Professor, Department of Physical Education, Faculty of Humanities, University of Ayatollah Alozma Boroujerdi, Lorestan, Iran
2 Ph.D., Department of Biochemistry, Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
3 Master of Science, Department of Physical Education and Sport Sciences, Islamic Azad University of Hamedan, Hamedan, Iran
*Corresponding Author: Saeed Daneshyar, Department of Physical Education, Faculty of Humanities, University of Ayatollah Alozma Boroujerdi, Boroujerd, Lorestan, Iran. Tel: +989037324793, Fax: 066432102, Email: s.daneshyar@abru.ac.ir

Abstract

Background and aims: Beta-adrenergic signaling deficiency has been established to be related to obesity and related diseases. Beta3- adrenergic receptor (Adrb3) and beta-arrestin2 (Barr2) are pivotal agents in the beta-adrenergic-signaling pathway. This study aimed to investigate the preventive effect of aerobic training on dysregulation of Adrb3 and Barr2 gene expression that was induced by high-fat diet (HFD) in inguinal white adipose tissue of mice.

Materials and Methods: Twenty-one C57BL/6 mice were assigned to three groups as follows: 1) control group (n=7), 2) high-fat diet-induced overweight (HFD-OW) (n=7), and 3) high-fat diet with aerobic training (HFD-AT) (n=7). The HFD-OW group were fed with a HFD for 12 weeks. The HFD-AT group had aerobic training for six weeks on a treadmill in addition to feeding with the HFD. The real-time polymerase chain reaction (PCR) method was used to measure the gene expression of Adrb3 and Barr2 in inguinal white adipose tissue.

Results: The gene expression of Adrb3 did not significantly change between groups (P>0.05). However, the expression of Barr2 in HFD-OW group was significantly increased as compared to the control group (1.5-fold: P=0.001). Interestingly, the Barr2 expression in HFD-AT group was significantly lower compared with HFD-OW group (P=0.045).

Conclusion: The results indicated that aerobic training could inhibit the upregulation of Barr2 induced by HFD. It seems that a portion of the preventive effect of aerobic training on the development of obesity may be mediated by inhibiting the Barr2 expression in adipose tissue.


Keywords: Obesity, High-fat diet, β-Arrestin, Exercise training, White adipose tissue

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 82

Your browser does not support the canvas element.


PDF Download: 58

Your browser does not support the canvas element.

Submitted: 18 Jan 2021
Accepted: 01 May 2021
ePublished: 29 Sep 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)