Submitted: 13 Mar 2018
Accepted: 21 Jul 2018
First published online: 04 Apr 2019
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - FireFox Plugin)

J Shahrekord Univ Med Sci. 2019;21(2):57-63.
  Abstract View: 72
  PDF Download: 80

Original Article

Antibacterial effects of vancomycin in combination with methicillin against methicillin-resistant and methicillinsensitive Staphylococcus aureus

Ali Abdolahi 1, Alireza Khodavandi 2 * ORCiD

1 Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran
2 Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran
*Corresponding Author: Alireza Khodavandi, Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran. Tel: 00987432332033, Email: alireza_khodavandi@yahoo.com, Email: khodavandi@iaug.ac.ir

Abstract

Background and aims: Staphylococcus aureus is considered as a frequent cause of infections. Therefore, antibacterial agents including β-lactam are normally used to treat these infections while the emergence of antibiotic resistance is one of the major clinical problems in this respect. Accordingly, combination antibacterial therapy is one way to alleviate this problem. As a result, the present study aimed to investigate the combined effects of vancomycin and methicillin on clinical isolates of methicillin-resistant S. aureus (MRSA) and methicillinsensitive S. aureus (MSSA). In addition, a series of follow-up studies were performed to evaluate the antibacterial activity.

Methods: The current cross-sectional study was conducted on 40 hospitalized patients from various clinical samples such as pus/wound swabs, blood, urine and sputum during a 6-month period in 2017. To this end, the antibacterial activities of vancomycin and methicillin, alone and in combination were investigated against MRSA and MSSA. Eventually, the inhibitory effects of vancomycin and methicillin alone and in combination were studied on the growth profile of MRSA and MSSA, as well as the expression of mecA gene.

Results: Based on the results, the significant synergistic and partial synergistic activity with fractional inhibitory concentration (FIC) and fractional bactericidal concentration (FBC) indexes ranged from 0.27-0.938 and 0.313-0.844, respectively, in the combination of vancomycin and methicillin in MRSA and MSSA isolates. Further, the obtained data indicated that the combination of vancomycin and methicillin had a synergistic effect against MRSA isolates (P<0.01). The expression levels of the mecA gene were down-regulated by 5.25- fold in the combination of vancomycin and methicillin in MRSA isolate (P<0.001).

Conclusion: In general, these events may reflect the potential uses of vancomycin and methicillin combination against MRSA. However, a greater understanding of the underlying molecular mechanisms of vancomycin and methicillin in combination could contribute to the development of therapeutic strategies.

First name
 
Last name
 
Email address
 
Comments
 
Security code


Article Viewed: 72

Your browser does not support the canvas element.


PDF Downloaded: 80

Your browser does not support the canvas element.