J Shahrekord Univ Med Sci. 2019;21(6): 280-283.
doi: 10.34172/jsums.2019.49
  Abstract View: 1132
  PDF Download: 380

Original Article

Determination of the carbapenem resistance in Escherichia coli isolated from samples obtained from Shahrekord hospitals and determination of their minimum inhibitory concentration

Farshad Kakian 1 ORCID logo, Behnam Zamanzad 2* ORCID logo, Abolfazle Gholipour 2 ORCID logo, Kiarash Zamanzad 3

1 Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Medical Bacteriology, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Student of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran
*Corresponding Author: Email: bzamanzaad@yahoo.com


Background and aims: Carbapenems are the final-line treatments for multidrug-resistant, gram-negative infections. The patterns of resistance to carbapenems among hospital bacterial pathogens vary widely across different hospitals in a country. Considering that Escherichia coli is one of the most important causes of nosocomial infections, it is essential to study its drug resistance.

Methods: In this descriptive-analytical study, a total of 80 samples of E. coli isolated from inpatients with urinary tract infections (UTIs) were collected in different wards (i.e., women, urology, infectious, and ICU) of Shahrekord hospitals. After the diagnosis and confirmation of bacteria by standard bacteriological methods, their sensitivity to imipenem and meropenem was investigated by the antibiogram (diskdiffusion) method. Then, the minimum inhibitory concentration (MIC) was determined by the E-test strip according to the Clinical and Laboratory Standards Institute (CLSI) standard.

Results: In this study, resistance to meropenem and imipenem by antibiogram (disc diffusion) was observed in 21 (25.26%) and 20 (25%) of the isolates, respectively. Twenty isolates had MIC ≥4 μg/mL for meropenem, 13 isolates demonstrated MIC≥4 μg/mL for imipenem, and 14 isolates had 1≤MIC<4 μg/mL and were semi-sensitive.

Conclusion: In general, E. coli had significant resistance to carbapenems. Therefore, rapid and accurate identification of these strains can be a major step to the treatment and control of these strains and prevention of the spread of the resistance

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Submitted: 14 Oct 2018
Accepted: 27 Jun 2019
ePublished: 31 Dec 2019
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