Logo-jskums
J Shahrekord Univ Med Sci. 2022;24(4): 196-202.
doi: 10.34172/jsums.2022.32
  Abstract View: 58
  PDF Download: 26

Original Article

The effect of hydro-alcoholic extracts of Nigella sativa, Carum carvi, Taraxacum officinale, and royal jelly on the survival of HDF cell line under H2O2-induced oxidative stress compared to metformin

Fatemeh Shirani –Lepri 1 ORCID logo, Kahin Shahanipour 1* ORCID logo, Ramesh Monajemi 2 ORCID logo, Golnoosh Rezaeizadeh 3 ORCID logo, Elahe Sadat Mousavi-Abnily 1 ORCID logo

1 Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
3 Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
Corresponding Author: Kahin Shahanipour, Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. Tel:+989132289324, Email: shahanipur_k@yahoo.com

Abstract

Background and aims: Highly active oxygen causes DNA or protein damage. This study aimed to determine the effect of the extract of three medicinal plants, royal jelly, and metformin on the survival of human dermal fibroblast (HDF) cells under oxidative stress induced by H2 O2 .

Methods: Hydroalcoholic extracts were prepared from Nigella sativa, Carum carvi seeds, and Taraxacum officinale flower. The growth curve of HDF cells was plotted in Dulbecco’s modified eagle medium (DMEM). The cells were exposed to H2 O2 stress and treated with different concentrations of the extracts, royal jelly, and metformin. The cells viability was then calorimetrically analyzed during different times in 69-well plates.

Results: The exponential growth phase of HDF cells was observed during 24–72-hour growth. High cell survivals were seen during 72 hours of exposure to 25 mg/mL royal jelly (99.13±3.22%), 15 mg/mL C. carvi extracts and N. sativa (97.87±3.25% and 96.22±5.47%, respectively), 10 mg/mL royal jelly (96.14±4.72%), 16 mg/mL metformin (91.23±5.34%), and 8 mg/mL T. officinale (86.31±7.44%), respectively. These results were significantly higher than the results of 48 and 24 hours of exposure to 15 mg/mL N. sativa (P=0.013), 15 mg/mL C. carvi (P=0.019), and 16 mg/mL metformin (P=0.027). Moreover, royal jelly (25 mg/mL) significantly indicated greater effect on the cell survival compared with the highest concentrations of the extracts and metformin at different exposure times (P=0.016-0.032).

Conclusion: Royal jelly, N. sativa, and T. officinale extracts revealed remarkable effects on the stressed HDF cells; therefore, they can be proposed for treatment of wounds and burns after clinical analysis.

Keywords: Plant extracts, Royal jelly, Metformin, Oxidative stress, HDF cell line
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 58

Your browser does not support the canvas element.


PDF Download: 26

Your browser does not support the canvas element.

Submitted: 25 Dec 2021
Revision: 10 Jul 2022
Accepted: 16 Jul 2022
ePublished: 08 Sep 2022
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)