Submitted: 15 Oct 2018
Accepted: 20 Nov 2018
First published online: 24 Jun 2019
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J Shahrekord Univ Med Sci. 2019;21(3):110-113.
  Abstract View: 45
  PDF Download: 71

Original Article

Comparison of polystyrene versus cycloolefin microplates in absorbance measurements in the UV/VIS region of the spectrum

Mehri Abedi 1 ORCiD, Reza Ahangari Cohan 2, Mehdi Shafiee Ardestani 3 * , Fatemeh Davami 1 ORCiD

1 Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
2 Department of Pilot Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran
3 Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
*Corresponding Authors: Fatemeh Davami, Biotechnology Research Center, Pasteur Institute of Iran, No. 69, 12th Farwardin Ave, Tehran, Iran, Post Code: 1316943551. Phone: +98 9126046412, Fax: +98 21 66480780, Email: f_davami@pasteur.ac.ir. Mehdi Shafiee Ardestani, Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, 16 Azar Ave, Enghelab Sq., Tehran, Iran, Post Code: 14155-6451. Phone: +98 912 8267629, Fax: +98 21 66959098, Email: Email: shafieeardestani@tums.ac.ir

Abstract

Background and aims: Polystyrene microplates are generally used to specify the absorption in the visible light region of the spectrum. However, they are capable of absorption in the ultraviolet (UV) range of the spectrum while they are not suitable for UV spectroscopy analysis. This study aimed to compare polystyrene and cycloolefin microplates for their background absorbance characteristics in the UV/ VIS region of the spectrum.

Methods: Background absorbance of the mentioned microplates was measured using two different spectrophotometers and four various samples.

Results: The analysis of our results verified the advantage of applying cycloolefin microplate over polystyrene one for absorbance measurements in the UV range of the spectrum.

Conclusion: In general, suitable microplate selection is a critical factor in absorbance measurements, especially in the UV portion of the spectrum.

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